Description: PolyMAG-100
Size (sufficient for n μg DNA): 100
Number of transfections (96-well format): 1000
Magnetofection™是一種簡單、高效的通過磁性微粒進(jìn)行培養(yǎng)細(xì)胞轉(zhuǎn)染的方法，提供兩類即用型試劑如下：
1. PolyMAG 普遍適用于核酸轉(zhuǎn)染至細(xì)胞的磁性微粒，通過一步操作可成功的將質(zhì)粒DNA、反義寡核苷酸和siRNAs轉(zhuǎn)入細(xì)胞。
2. CombiMAG 可與其他轉(zhuǎn)染試劑如聚陽離子和脂質(zhì)結(jié)合使用的磁性顆粒，可用于質(zhì)粒DNA、反義寡核苷酸和siRNAs和病毒。
Magnetofection™ is a novel, simple and highly efficient method to transfect cells in culture. It exploits magnetic force exerted upon gene vectors associated with magnetic particles to draw the vectors towards, possibly even into, the target cells. In this manner, the full vector dose applied gets concentrated on the cells within a few minutes so that 100% of the cells get in contact with a significant vector dose. This has several important consequences:
Greatly improved transfection rates in terms of percentage of cells transfected compared to standard transfection.
Up to several thousand fold increased levels of transgene expression compared to standard transfections upon short-term incubation.
High transfection rates and transgene expression levels are achievable with extremely low vector doses, which allows to save expensive transfection reagents.
Extremely short process time. A few minutes of incubation of cells with gene vectors are sufficient to generate high transfection efficiency, compared to several hours with standard procedures.
Storage:
All components of the Magnetofection™ kit should be stored at room temperatur (20-25°C). After first use store the kit at +4°C.
Do not freeze the magnetic nanoparticles
Do not add anything to the stock solution of magnetic nanoparticles
Shipping Conditions: Room Temperature
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